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Appendix 1: Evaluation of Commercially Available Analytical Methods for Proteins from Major Food Allergen

Manufacturera

Method

Validationb

Elisa Systems

Enhanced Casein Alert for Total Milk

No

Neogen

No

Neogen

Veratox for Total Milk

No

R-BioPharm

RIDASCREEN Beta- Lactoglobulin Milk Residue

No

SafePath

No

Tepnel

BioKits BLG

No

Tepnel

BioKits BSA

No

Tepnel

BioKits Casein

No

Tepnel

BioKits Casein Rapid

No

Elisa Systems

Egg

No

Neogen

Alert for Egg

No

Neogen

Veratox for Egg

No

5 whole

No

milk 10 dry nonfat milk Not reported

2.5

Egg

Sensitivity

Quantitation

(LODc)

(LOQc) (ppm)

(ppm)

1

1

0.2

5

Not reported

Not reported

7.5

25

10

25

1

2

Not reported

Not reported

0.5

1

5

N

Not reported

2.5

Allergen

Raw and Processed

Species Specificity

Protein(s) Detected

Practicality

Foods? Yes

Not reported

Casein

Yes

Yes

Not reported

Not reported

Yes

Yes (for specified foods) Not reported

Not reported

Not reported

Yes

Not reported

Beta- Lactoglobulin

Yes

Not

Not reported

reported

Yes

Not reported

Yes

Not reported

Yes

Not reported

Yes (for specified foods) Yes

Yes (for specified foods) Yes (for specified foods)

Not reported

Ovalbumin, Ovamucoid Not reported

Not reported

Beta- Lactoglobulin Primarily Beta- Lactoglobulin Bovine Serum Albumin Primarily Alpha-Casein Casein

Yes

Yes

Yes

Yes

Yes

Yes

Not reported

Not reported

Yes

Not reported

Yes

Revised Threshold Report

Page 86 of 108

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