We use the JAK2 MutaQuant™ Assay from Ipsogen. This highly sensitive approach in- volves the use of a SNP specic forward primer resulting in the selective amplication of mutant or wild type allele. The uorescent labeled products are easily detectable on a real-time PCR instrument. The quantities of normal and mutated JAK2 copies are measured using a standard curve and the relative percentage of genes that possess this mutation detected (%V617) is reported.
The JAK2 MutaQuant™ assay is designed to detect less than 1 copy of JAK2 V617F mutation out of 100 normal copies of the gene, or a sensitivity of <1%. The variation of this assay though can be approximately 5% between laboratories and within a laboratory.
PLEASE NOTE SAMPLE SHOULD BE RECEIVED SAME DAY OR SHIPPED OVERNIGHT ON ICE (DO NOT FREEZE)
5-10mls peripheral blood OR 3-5mls bone marrow in EDTA tube
Please contact laboratory for pricing
83890, 83896, 83898, 83907, 83912, 83914
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