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TESTING METHODOLOGY:

We use the JAK2 MutaQuant™ Assay from Ipsogen. This highly sensitive approach in- volves the use of a SNP specic forward primer resulting in the selective amplication of mutant or wild type allele. The uorescent labeled products are easily detectable on a real-time PCR instrument. The quantities of normal and mutated JAK2 copies are measured using a standard curve and the relative percentage of genes that possess this mutation detected (%V617) is reported.

ACCURACY:

The JAK2 MutaQuant™ assay is designed to detect less than 1 copy of JAK2 V617F mutation out of 100 normal copies of the gene, or a sensitivity of <1%. The variation of this assay though can be approximately 5% between laboratories and within a laboratory.

SPECIMEN:

PLEASE NOTE SAMPLE SHOULD BE RECEIVED SAME DAY OR SHIPPED OVERNIGHT ON ICE (DO NOT FREEZE)

5-10mls peripheral blood OR 3-5mls bone marrow in EDTA tube

TURN-AROUND TIME:

7-10 days

COST:

Please contact laboratory for pricing

CPT CODES:

83890, 83896, 83898, 83907, 83912, 83914

REFERENCES:

  • 1.

    James C et al. (2005). Nature 434:1144.

  • 2.

    Kralovics R et al. (2005). N Engl J Med 106:1779.

  • 3.

    Lacout C, et al. (2006). Blood 108:1652.

  • 4.

    Delhommeau F, et al. (2007). 109:71.

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