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Sampling Tree Swallow Eggs and Nestlings TVA-KIF-SOP-28

March 2010

    • g.

      Label the jar on the side and lid and seal with a labeled custody seal.

    • h.

      Freeze processed samples at < -10ºC until shipment to the approved laboratory for analysis.

    • i.

      Complete the Tree Swallow Egg Field Specimen Collection/ Processing form (Table 3) documenting custody and sample integrity which accompanies the samples from the time of collection through temporary storage awaiting shipment to the laboratory.

    • j.

      For shipping, place frozen samples in a cooler with dry ice and ship to the approved laboratories in accordance with the Sample Labeling, Packing, and Shipping SOP (TVA-KIF-SOP-07).

    • k.

      Complete a COC form which accompanies the samples from time of collection through shipment to the laboratory. Refer to Field Documentation SOP (TVA- KIF-SOP-06) for proper COC procedures.

      • 3.5.2

        Nestling Sample Processing Nestlings are processed in a similar manner as the egg samples.

        • a.

          Remove each sample from the collection container and resealble bag.

        • b.

          Weigh nestling on a digital analytical scale (0.001 g).

        • c.

          Measure the length of the tarsus (on leg) and third primary feather using a digimicrometer (0.01 mm).

        • d.

          Record measurements on the Tree Swallow Nestling Field Specimen Collection/ Processing form (Table 4).

        • e.

          Transfer information from original resealable plastic bag to a new bag.

        • f.

          Place specimen in the new bag; label and seal bag with a tamper-evident custody seal, and temporarily store in a cooler with dry ice.

        • g.

          Once samples are processed, freeze samples at < -10ºC for shipment to the approved laboratory.

        • h.

          Complete the Tree Swallow Nestling Field Specimen Collection/Processing form (Table 4) to document custody and sample integrity which accompanies samples from time of collection through temporary storage awaiting shipment to the laboratory.

        • i.

          For shipping, place frozen samples in a cooler with dry ice and ship to the approved laboratories in accordance with the Sample Labeling, Packing, and Shipping SOP (TVA-KIF-SOP-07).

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This document, in part or in whole, is the property of the Tennessee Valley Authority (TVA) for sole use by TVA employees or TVA contractors or as otherwise consented to in writing by TVA.

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