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Field stop and aperture

stop units

for easy attachment

of laser and

other special illuminators. Removable field stop and

aperture

stop and

units facilitate

detachment

of

simple attachment laser and

ILLUMINATION VERSATILITY

conventional

ultraviolet

(UV) excitation

are both required when fluorescence

microscopy staining. switching

moves toward multi- However, this requires the light source from the

conventional mercury halogen source. The

burner to twin-lamp

a

housing configuration makes the switchover

on the AX70 between the

mercury burner and halogen source simple operation.

a

Quick excitation

  • -

    a convenient

light switchover feature for various

photometry applications.

and measurement

Working in combination

OSPlOO-DNA

photometry

with the system, the

additional

illuminators.

This versatility

permits application-specific

incident

light illumination.

In particular,

it

AX70 easily

handles

measuring

methods

fluorescent

indicators

different using several

These

range

enables

laser illumination

manipulation

and caged

release

photoactivation.

for laser compound

from

1-wavelength

excitation/

l-wavelength 2-wavelength

photometry

(1 Exl Em)

excrtatiorGwavelength

to

Twin-lamp housing configuration

photometry AX70IOSPI

(2Ex2Em).

Thus, the

00

combinatron

can

with a quick, changeover.

one-touch

illumination

measure fluorescence single wavelength with

intensities at a dual-wavelength

Two lamp housrngs srmultaneously onto signrfrcantly expand

potential

Infrared

can

be mounted

the

AX70 to

the

mrcroscope’s

(IR)

excitation

and

excrtatron Indicator.

using the fura-

or

These

advantages

BCECF make It

ideal for analyzrng

rntracellular calcrum

ion concentrations

and pH values

PAF (Photoactivated The PAF technique

Fluorescence) lrradlates caged compound

with an ultravIolet

ray, Instantly transforming

them to onginal PAF also allows

active substances

control of intracellular

The induced

reactlon

IS then measured

reactlon time and locatlon

and examined

Applications *Examining

muscle

contraction

due

to

increased

intracellular

Ca2*

concentrations *Study of cytoskeleton

assemblies

*Ion channel research *Study of receptor-bonding

of

neural

transmitters

and their functions

AX70 -the The AX70 sideways

obvious

choice

incorporates hm~mat~on

a vertical capabhfy

illuminator

wrth

The Y-shape also provides

an uncluttered

work surface for conveniently

positioning

an injector or other

upright microscope (with samples usually

IS required measuring

ancillary

equipment

to observe

internal

100

  • -

    200pm

thick)

near the stand

structures

of

An cells

10

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