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MRMs

Quad Mass Filter (Q1)

Ions

165

268

280

170

210

250

290

210

210

222

190 210

Spectrum with

Q1 lets

background

target ion 210

ions (from ESI)

pass through

Quad Mass Filter (Q3)

Collision Cell

158

191

210

150

170

190

210

160 190 no chemical background

Collision cell breaks ion 210 apart

Q3 monitors characteristic fragments 158 and 191 from ion 210 for quant and qual.

158

191

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