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Columns and Gradients

y The column is a ZORBAX Eclipse Plus C18, narrow bore RR 2.1x100mm 3.5 micron column. Part number 959793 902.

y Group 1: The gradient is from 10% acetonitrile, 90% 0.1 % formic acid in water, held for 5 minutes at 0.2 mL/min, then to 0.3 mL/min in 1 minute. In 24 min go to 60% ACN and finally to 100% ACN in 30 minutes.

y Group 2: The gradient is from 10% acetonitrile, held for 10 minutes, and then to 100% ACN in 30 minutes at a flow rate of 0.2 mL/min.

y Group 3: The gradient is from 40% acetonitrile/methanol and 60% water (0.1% ammonium acetate+0.1% Acetic Acid) to 100% ACN/MeOH in 12.5 minutes at a flow rate of 0.2 mL/min.

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