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M J Larkin  Biological Sciences. The  Queen’s University of Belfast.

Genomics and Metagenomics

Chain termination sequencing used for genomes to date – 800 bps per read

Pyrosequencing “454” direct sequencing of single strands – 300 bps per read – but rapid.

Use in analysis of RNA transcipts

Use for  rapid analysis of ALL DNA in environment – metagenomics

Screening environment for useful genes.

Expression requires suitable host

E.Coli not always suitable

Other hosts more useful – e.g Rhodococcus – used in many industrial processes

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