M J Larkin Biological Sciences. The Queen’s University of Belfast.
Genomics and Metagenomics
Chain termination sequencing used for genomes to date – 800 bps per read
Pyrosequencing “454” direct sequencing of single strands – 300 bps per read – but rapid.
Use in analysis of RNA transcipts
Use for rapid analysis of ALL DNA in environment – metagenomics
Screening environment for useful genes.
Expression requires suitable host
E.Coli not always suitable
Other hosts more useful – e.g Rhodococcus – used in many industrial processes