Antiseptic agents: Disinfectants that are applied on animate bodies.
Characteristics: . Never be toxic to cells . Never be corrosive . Should never change nature of skin Eg. Savlon Alcohol( 70%) Iodine tincture Iodophor
Physical methods of sterilization and disinfection
Heat: the most reliable and universally applicable method of
Mechanism of action . Dry heat – denatures protein. . Moist heat – denatures and coagulates protein.
Dry heat : It is less efficient and requires high temperature and long period heating than moist heat. Dry heat can be used by the following methods:
Incineration : It is an efficient method of sterilization and disposal of contaminated needles, syringes and cover slips at high temperature
Red heat : Inoculating wires, loops and points of forceps are sterilized by holding them in the flame of a Bunsen burner until they are red hot. 99