NB: Most culture media are sterilized at a pressure of 15 lb/in2, at a temperature of 121 OC for 15 minutes.
At the end of sterilizing time, turn off the heat
and allow the autoclave to cool naturally.
It destroys bacterial endospores and vegetative cells. Uses: Sterilize solid and fluid culture media, gowns, medical and surgical equipment.
Time –Temperature-Pressure level relationship in moist heat sterilization (autoclaving)
Tempe 121 0c 126 0c
Methods of controlling sterilization
Recording of temperature and time of each sterilizing cycle.
Heat-sensitive autoclave tape fixed to the outside of each
pack. . Color change of autoclave tape from blue to brown-black indicates completesterilization. 3. Biological indicator : Use of paper strips impregnated with
spores of Bacillus stereothermophilus . Put the paper strip in the culture medium after autoclaving and observe for germinating bacteria to check for growth. In complete sterilization there should not be bacterial growth.