In a hospital with a microbiology Laboratory.
Give the patient a clean (need not be sterile), dry, wide- necked, leak- proof container and request him or her to cough deeply to produce a sputum specimen Note: The specimen must be sputum, not saliva. Sputum is best collected in the morning, soon after the patent wakes and before any mouth-wash is used. If the patient is young child and it is not possible to obtain sputum, gastric washings can be used for the isolation of M.tuberculosis but not for other respiratory 1.
Label the container and fill the request form.
If pneumonia or bronchopneumonia is suspected, deliver the
sputum to the laboratory with as little delay as possible.
Because organisms such as S.pneumoniae and H. influenza require culturing as soon as possible. If the specimen is for the isolation of M.tuberculosis, ensure it is delivered to the laboratory within 2 hours or kept at 4 Oc until delivery is possible. If pneumonia plague is suspected: Deliver the
sputum to the laboratory as soon as possible. sure that the specimen is marked HIGH RISK.