For dispatching to a microbiology laboratory Collect the sputum in a container as usual. Depending on whether the sputum is for the isolation of pneumonia and bronchopneumonia or M.tuberculosis, proceed as follows:
Pneumonia and Bronchopneumonia pathogen
Collect a purulent part of the sputum on a cotton wool swab, and insert in a container of Amies transport medium. Label the container using a lead pencil
Amies will help the pathogens to survive and from being overgrown by fast-multiplying commensas.
Make a smear on slides for gram staining and fix using heat or alcohol and send the swab and the request form to reach to the laboratory.
Make a smear of the sputum on slides for Ziel-
Neelsen staining, from the most pumlent materials.
Fix with alcohol.
If pneumonia plague is suspected:
Send the swab of the sputum in cary-Blair transport medium to reach the microbiology laboratory. Caution: Y. pestis is highly infectious organism.