Laboratory examination of sputum
Describe the appearance of the specimens
Purulent: Green- looking with pus and mucus.
Mucopurulent: Green- looking with pus and Mucus
Mucoid: Mostly mucous
Mucosalivary: Mucus with a small amount of saliva
Bloody: should be reported.
Examine the specimen microscopically. Gram smear
Look for pus cells and bacteria
Gram positiveve diplococci that could be S. pneumoniae
Gram positive cocci that could be S. aureus
Gram negative rods that could be H. influenzae
Gram negative capsulated rods that could be K.
Additional investigation Look for:
saline preparation, if paragonimiasis is suspected
Eosin paration, if asthma or other allergic condition is
suspected. Potassium hydroxide (KoH) preparation, infection is suspected (yeast cells, Nocardi
if fungal a species,