Giemsa smear, if histoplasmosis or pneumonic plague
3) Culture the specimen
To obtain as pure a culture as possible of pathogen it is necessary to reduce the commensals inoculated.
a respiratory number of
Ways of reducig commensal numbers include washing the sputum free from saliva or liquefying and diluting it.
agar and chocolate agar Wash a purulent part of the sputum in about sml of sterile physiological saline. Inoculate the washed sputum on plates of blood agar and chocolate (heated blood) agar. Add an optochin disc to the chocolate agar plate. This will help to identity S. pneumonia. Incubate the blood agar plate aerobically and the chocolate agar in a co2 enriched atmosphere at 35-370c for upto 48 hours, examining for growth atfter ovemight incubation.
Additional: Lowenstein suspected.
About 20 minuets before culturing, decontaminate the
specimen by mixing equal volumes of sputum and