sodium hydroxide (NaoH) 40 g/l or ( 4% W/v ) solution. Shake at intervals to homogenize the sputum. Using a sterile pasteur pepitte, inoculate 200 Nl (0.2ml) of the well-mixed homogenized sputum on a slope of acid Lowenstein Jensen medium. Allow the specimen to run down the slope. Incubate at 350c –370c in a rack placed at an agle of about 450c to ensure that the specimen is incontact with
the full length of the slops.
After one week, and continue to groth.
place the slope in an upright position incubate, examining twice a week for
4. Examine and report the culture
Examine the blood agar & Chocolate agar culture for:
Pse. Aeruginosa Examin Lowenstein Jensen e culture for: M.. TUBERCULOSIS