Incubate the plate aerobically
a moist atmosphere container with a wet
piece of cotton wool) at 35-370c for up to 6 days,
Examining for growth after about 48 hours incubation.
Culture of swab received in bordetella transport medium:
Inoculate the swab on a plate of CCBA
Return the swab in its container and incubated it at 35- 370c
Examine the plate for growth after 48hoursrs incubation.
If no growth is seen, inoculate a second plats of CCBA with the incubated swab.
Reincubate the first plats for a further four nights.
Examining for growth every 24 hours.
The second plate for up to 6 days.
2. Examine and report the cultures
Blood agar and chocolates agar cultures(routine)
Look for coloniess that could be
H. influenzae Neissena mengitdis
pyogenes (Group A)