Collection and Transport of Ear Discharges
Collect a specimen of the discharge on a sterile cotton.
Place it in container of Amies transport medium, breaking off the swab stick to allow the bottle top to be replaced tightly.
Make a smear of the discharge on a slide (for Gram staining).
Label the specimens and send them with its request form to the laboratory Within 6 hours.
Laboratory examination of Ear Discharges
culture the specimen
Blood agar and Macconkey agar
Inoculate the specimen on blood agar and macconkeg agar Incubate both plates aerobically at 35-370c overnight. Additional: Chocolate agar if the patient is a child: Inoculate the specimen on chocolate (heated blood) agar for the isolation of H. influenza. Incubate the plate in a carbon dioxide enriched atmosphere at 35-370c for up to 48 hours, examining for growth after overnight incubation.