Blood agar (Kanamycin) for anaerobic incubation if the infection is chronic
Inoculate the specimen on blood agar, preferably that which contains Kanamycin to inhibit the growth of commensals.
Incubate the plate anerobically for up to 48hours, checking for growth after overnight incubation.
Sabouraud agar if a fungal infection is suspected
Inoculate the specimen on sabouraud agar, and incubate at room tempreture for up to 6 days.
Examine the specimen Microscopically
Make an evenly spread of the specimen on a shide.
Allow the smear to air-dry in a safe place.
Fix and stain by Gram technique.
Look for pus cells and bacteria
Gram positive cocci that could be S.aureus
Gram positive streptococci or diplococi that could by streptococci pathogens