Gram negative rods that could be
influenzae, p. aeroginosa, klebsiella specis, proteus species, E.coli or other s.
Gram positive yeast cells that could be candida species.
Small numbers of Gram positive cocci, streptococci, rods and also Gram negative rods may be seen in smears of ear discharges because these organisms form part of the normal microbial flora of the external ear.
Potassium hydroxide preparation if a fungal infection is suspected
Mix a small amount of the specimen with a drop of potassium hydroxide, 200g/l (20%W/v) on a slide, and cover with a coverglass.
After 10 minutes, or when the preparation has cleared sufficiently, examine microscopically using 10x or 40x objective.
Brnaching septate hyphae with small round spores, that could be Aspergillns speies
Pseudohyphae with yeast cells, that could be candida specis (Gram positive)