Specimen from the eye must be cultured as soon as possible after collection because the natural secretions of the eye contain antibacterial enzymes.
Using a dry sterile cotton wool swab, collect a specimen of discharge (if an inflant, swab the lower conjunctival surface).
Inoculate the discharge on the following culture media:
Chocolate (heated blood) agar
MNYC N. gonorrheae selective medium, if the patient is a newborn infant.
Make a smear of the discharge on slide (frosted-ended) for staining by the Gram technique. If C. trachomatis infection of the newborn is suspected, make a second smear for staining by thr Giemsia technique and label the plate and slide.
As soon as possible, deliver the inoculated plates and smear(s) with request form to the laboratory.
Laboratory examination of the eye specimen 1. Culture the specimen Routine:
agar and chocolate agar Inoculate the eye discharge chocolate (heated blood) agar.