Gram positive streptococci or diplococci that could be
G ram positive cocci that could be S.aureus
Gram negative rods that could be Haemophilus species.
Additional Giemsa smear if C. trachomatis infection is suspected:
Fix the air-dried smear by covering it with methanol for 3 minutes.
Dilute the Giemsa stain in the buffered water
trachomatis, dilute the stain 1 in 40:
Fill asmall cylinder to the 19.5 ml (mark with buffered water)
Add 0.5ml Giemsa stain to 20ml mark. Eg. 0.5ml + 19.5ml For Other organisms, 1ml + 19ml
Stain the smear with diluted Giemsa in dish
trachomatis, stain 1 ½ - 2 hours For other organisms, stain 25-30 minutes
Wash the slide from the dish and rise the smear
with buffered water. And let the smear to air-dry and examine with oil immersion objective.
trachomatis inclusion bodies ---------- Blue-mauve to dark purple. Depending on the stage of development; If the inclusion body is more mature, it will contain ---- red- mauve stiaing elementary particles.