Gram positive Staphylococci Micrococci Anaerobic cocci Viridans streptococci Enterococci Dephtheoids
Gram negative Escherichia Coli and other coliforms
Collection of skin specimens and ulcer materials
Using a sterile dry cotton wool swab, collect a sample of discharge from the infected tissue. If there is no discharge, use swabmoistened with sterile physiological saline to collect a specimen. Insert the swab in a sterile tube.
If the tissue is deeply ulcerated and necrotic (full of dead cells); Aspirate a sample of infected material from the side wall of the ulcer using a sterile needle
and syringe. Fluid from
blisters: Aspirates a
specimen using a sterile needle and syringe. Serous fluid from skin ulcers, papillomas or papules, that may contain treponemes:
Collect a drop of the exudates directly on a clean cover glass and invert on a clean slide.