Specimen required for diagnosis of gonorrhoea
Male patients: . Smears of urethral discharge . Rectal swab from homosexual patient Female patients . Smears of mucopus from the cervix and urethra Note: N. gonorrhoeae infects the mucaus membranes of the cervix, not the vagina. The pathogen is, therefore, more likely to be isolated from a cervical swab than from a vaginal swab.
Collection of Urethral specimens
cleans the urethral opening using a swab moistened with sterile physiological saline.
Gently massage the urethra from above downwards, and collect a sample of pus on a sterile cotton wool swab.
The patient should not have passed urine preferably for 2hours before the specimen is collected.
Make a smear of the discharge on a slide for staining by the Gram technique and label the specimen.
Collection of Cervical specimen
Moisten a vaginal speciemen with sterile warm water and insert into the vagina.
Cleause the ceroix using a swab moistened with sterile physiological sahrie.
Pass a sterile cotton wool swab into the endocervical canal and gently rotate the swab to obtain a specimen.