Look for Gram negative coccobacilli showing bipolar staining
Additional culture Blood agar (aerobic and anaerobic), macCokey agar,and cooked meat medium, if puerperal sepsis or septic abortion is suspected Sabourand medium, if vaginal candidiasis is suspected and yeast cell not detected microscopically Serum culture, if chancroid is suspected ⇒H. ducreyi Microscopy Sahlie preparation, if trichomoniasis is suspected. Gemsa stained smear: If donovanosis is suspected Dark field preparation, if syphilis is suspected.
Colleciton, transport and examination of cerebrospinal fluid Possible pathogens
agalactiae (Group B)
Listeria monocytogenes Bacillus anthracis
Gram negative Neisseria meningitides H.influenzae type b