Chocolate (heated blood) agar
n a CO2 enriched atmosphere at 35-370C for 48hours,
cheeking for growth after overnight incubation.
Additional MacConkey and blood agar if the patiente is a newborn infant incubate both plate at 35-370C overnight
E.coli or other coliform
S. agalacteae (Group B)
Lowenstein Jensen medium if tuberculous meningitis is suspected Sabourand agar if cryptococcal meningitis is suspected. If capsulated yeast cells are seen in the microscopial preparations, inoculate a plate of sabouraud agar. Incubate at 35-370C for up to 72hours, cheeking for growth after overnight incubation.
5. Microscopy The microscopical examination of C.S.f is required if the specimen appears abnormal, contains cells and, or, the total protein is raised with a positive pandy’s test.