Gram negative intracellular diplococci that could be
Gram positive diplococci or short streptococci that could be S. pneumoniae. It is often possible to see the capsules as unstained are as around the bacteria.
Garm negative rods, possibly H. influezae
Gram negative rods, could also be E.coli or other coliforms, especially if the C.S.f is from a newborn infant.
Gram positive cocci in groups and singly, possible
Gram positive streptococci, possibly S. agalacteae (G -.B)
Gram positive yeast cells, C. neoformans.
Additional Ziel-Neelsen – smear
M. tuberculosis Indian ink preparation if cryptococcal meningitis is suspected: Wet preparation to detect amoebae or Trypanosome Giemsa stain to detect morulla cells or Burkett’s lymphoma cells
These can be found when trypanosomes have invaded the CNS.
They are larger than most lymphocytes, dark red mauve and contain vacuoles.