growth and isolation of as wide a range of pathogens as possible. The following media are suitable for routine culture of blood and bone marrow:
- - Tryptone medium Thioglycollate broth medium soya (tryptic soy)
Tryptone soya (tryptic soy) diphasic medium
agar slope with a broth medium. Because the bacteria can be seen growing on the slope, the need to subculture on a solid medium every few days is avoided, thus reducing the risk of contamination.
Tryptone soya diphasic medium consists of a tryptone soya
agar slope and a tryptone soya broth to which is added Liquoid and P-aminobenzoic acid. Liquoid: It is the commercial name for sodium polyanethol sulphonate. It prevents clotting of the blood and neutralize the natural bactericidal substances in fresh blood.
P. Aminobenzoic acid: This neutralizes the sulphonamides should these be present in the blood.
Tryptone soya diphasic medium is suitable for the growth of
a wide range of pathogens. Incubation in CO2 is required for the culture of brucella species. Strict anaerobes will not grow in this medium.