Thioglycollate broth ¾ This consists of a nutrient broth to which is added thiogiycollate to provide the condition necessary for the growth of anaerobes. Most aerobic bacteria will also grow in thioglycollates broth. ¾ Because liqoid is not added to this medium, a sufficient volume of broth must be used to prevent the blood from clotting and to dilute out the blood’s natural bactericidal substances. The blood should be diluted at least 1 in 10 in the broth.
Examination of Blood and Bone marrow 1. Collect and culture the specimen Blood
It should be collected before antimicrobial treatement has been started and at the time the patient’s temperature is beginning to rise.
To increase the chance of isolating a pathogen, it is usually recommended that at least two specimens (collected at different times) should be cultured.
Blood for culture must be collected as aseptically as possible.
Insert the needle through the rubber line of the bottle cap
and dispense 5ml of blood into each culture bottle. 3. Gently mix the blood with the bruch.