2. Clostridial food poisoning It causes secretory diarrhea due to release of enterotoxin in the intestine
Self-limiting diarrhea similar to that produced by B. cereus Laboratory diagnosis: Specimen: Infected tissue, pus, vomitus, left over food, serum Smear: Non-motile, capsulated, thick brick-shaped gram- positive rods in smears from tissue; spores are rarely seen.
1. Blood agar medium . β-hemolytic colonies are seen in blood agar in anaerobic atmosphere. . Some strains produce double zone of hemolysis. Cooked meat medium(Chopped meat-glucose medium) Thioglycolate medium 2.
. Saccharolytic property showing reddening of the meat with a rancid smell due to carbohydrate decomposition.
. Proteolytic property showing blackening of the meat with unpleasant smell due to protein decomposition. . Formation of gas Biochemical reaction: . Nagler reaction: Lecithinase C activity- Opacity in the egg-yolk medium due to lecithin break down