Streak colonies of C. perfringens on egg-yolk agar.
Cover half of the medium with C. perfringens antitoxin.
Look for dense opacity production by the growth of
perfringens; but no opacity on the area with antitoxin.
. Lactose fermentation: Reddening of the medium; red colonies when exposed to air.
. Litmus milk medium: “stormy- clot” formation due to acid and gas formation. Identification of C.perfringens rests on colony form, hemolysis pattern, biochemical reaction, and toxin production and neutralization by specific antisera.
Prompt and extensive wound debridement Polyvalent antitoxin Prevention and control
Early adequate debridement