TCBS (thiosulphate citrate bile salt sucrose agar)
media Selectivemedia V.cholerae.
. Observe for large yellow sucrose-fermenting colonies after 18-24 hrs of incubation.
Alkaline peptone water: Enrichment media for
Growth on and just below the surface of peptone water with in 4-6 hours at room temperature as well as 37 oc. Biochemical Reaction: . Oxidase-positive. . Ferment sucrose and maltose(acid; no gas). . Do not ferment L-arabinose. Treatment: Sensitive to tetracycline and chloramphenicol. Fluid and electrolyte replacement are the first line of management for cholera.
2.4.4. GENUS: CAMPYLOBACTER
Characteristics: . Small, delicate, spirally curved gram-negative bacteria. . Motile bacteria with single polar flagellum. . Stricly microaerophilic bactria requiring 5-10% o2 and 10% co2 enriched environment. . Oxidase and catalase positive.
Species of medical importance: