Diagnosis of anaerobic infection 1. Clinical . Foul smelling discharge due to short chain fatty acid products of anaerobic metabolism . Proximity to a mucosal surface . Gas in tissue due to production of co2 and H2 Lab. diagnosis . Grow most readily in complex media (Trypticase soy agar, Schaedler blood agar, Brucella agar, Brain-heart infusion 2.
agar) incubated at 35-37 enriched with CO2
0c in anaerobic atmosphere
Identificat ion of anaerobes is based on:
. Colony morphology . Pigmentation . Fluorescence . Biochemical reaction . Fatty acid production Treatment: Antimicrobials + Clindamycin Metronidazole Cefotetan Surgery