. Elicits the tuberculin reaction and antibody production. Polysaccharides . Induce the immediate type of hypersensitivity. Clinical manifestation: Incubation period: 4-6 weeks. Source of infection: Tuberculous patients Route of infection: Respiratory- Inhalation of droplet nuclei Ingestion of infected milk Disease: Pulmonary and extrapulmonary tuberculosis The disease generally manifests with low-grade persistent fever, night sweating, significant weight loss, fatigue and generalized weakness. Laboratory diagnosis: Identification of M. tuberculosis Specimen: Sputum; pleural, peritoneal and cerebrospinal fluid Smear: Acid fast bacilli from primary specimen. Cord forming acid-fast bacilli from culture. Culture: 3.
Semisynthetic agar media . Middle brook 7H10 and 7H11 . Used for observing colony morphology, susceptibility testing, and as selective media
Inspissated egg media . Lowenstein-Jensen medium It is the ordinary selective media for tubercle bacilli Raised, dry, cream colored colonies of tubercle bacilli after 3-6 wks of incubation