1.Flocculation test –VDRL , RPR . Positive after 2-3 wks of untreated syphilitic infection . Positive result revert to negative with in 6- 18 months of effective therapy of syphilitic infection Principle: Antigen and antibody (Reagin) reaction results in clumping after aggitation . It can give quantitative results, and valuable in establishing a diagnosis and in evaluating effect of treatment 2. Complement fixation test: Wasserman test; Kolmer test Principle: Reagin-containing sera (mixture of IgM and IgA) fix complement in the presence of “cardiolipin-cholestrol-lecithin complex” antigen. NB: Flocculation and Complement fixation tests are valuable in establishing diagnosis
and in evaluation effectiveness.
False-positive results in both tests can occur since the tests are non-specific.
Treponemal antibody tests . Fluorescent treponemal antibody-absorption test (FTA-abs) . Treponema pallidum- particle agglutination test (TP-PA)