Fluorescein-conjugated antibodies staining Immunohistochemical staining Giemsa staining Culture: Cultured in fletcher’s semisolid media Growth in the media is slow, requiring incubation for at least 8 wks Animal inoculation: Intraperitoneal inoculation of young hamsters with spirochetal contaminated fresh plasma or urine Demonstration of spirochetes after few days in peritoneal cavity Serology: EIA/ Agglutination tets High titers of agggglutinating antibodies after 5-8 wks of leptospiral infection Treatment:
Doxycycline Ampicillin/Amoxicillin Prevention and control:
. Preventing exposure to potentially contaminated water . Reducing contamination by rodent control . Chemoprophylaxis: Using doxycycline exposure during heavy
2.7. GENUS: RICKETTSIAE
Characteristics: .Obligate intracellular pleomorphic gram-negative coccobacilli occurring in single, pairs, short rods and filaments. . Poorly stained in gram reaction. . The organism stains red in macchiavello’s stain.