1.0ml into a tube containing 9ml of the of the BPW, mix with a fresh pipette, and
From the first dilution, transfer with the same pipette 1.0ml to 2nd dilution tube containing 9ml of the BPW, mix with a fresh pipette, and
Repeat using a 3rd, 4th tube or more until the required numbers of dilutions are made.
Shake all dilution carefully.
homogenate and of each dilution of the homogenate into each of the
Pour into each petri dish 15ml of the PCA (kept at 45 ±10c in water bath ) within 15 minute of the time of original dilution.
Mix the sample dilution and agar medium thoroughly and uniformly; allow to solidify. 360