Motility medium: inoculate 4 tubes by stabbing. Diffuse circular growth occurs after 24hours incubation at 350C.
Make a Gram stain from growth on TSA slant.
Halophilic nature: inoculate 4 tubes of STB containing 0, 6,8and 10% NaCI, incubate; V.parahaemolyticus will grow well in 6 and 8% NaCI but not in 0 and
e) f) g)
10% concentrations. MR-VP test Indole test Carbohydrate fermentation:
one tube each of glucose, lactose, sucrose, maltose, mannitol, etc. from TSA saint. After incubation check for acid production. Glucose fermentation: stab 2 subes of HLGB medium, overlay one tube with sterile paraffin oil and incubate for 2 day at 35 oc. yellow coloration of both tubes indicates fermentation, in the tube without oil only indicates oxidation.
V.paraphaemolyticus is fermenter producing no gas.
Cytochrome oxidase test: Allow 2-3 drops of alphanaphthol solution to flow over a fresh slant of V.parahaemolyticus