positive dilutions of GSTB and apply the 3 tube MPN table for final enumeration of the organism.
ENUMERATION OF BACILLUS CEREUS Principle This method is based on surface plating technique using a medium containing egg yolk on which the colonies of B. cereus are recognized by being surrounded by zones of turbidity. Apparatus and Glassware
Pipettes, 1 ml
Incubators, 200C, 300C, 350C
Culture media and reagents
a. b. c. d. e. f. g .
Buffered peptone water KG agar Nitrate broth Nutrient agar Nutrient gelatin VP medium Gram stains
Procedure Preparation of food homogenate Prepare as described as above Dilution Prepare as described above. Inoculation