Sodium chloride 5.0 gm Distilled water 1000 ml
Mix the ingredients and dissolve them by heating in a steamer. When cool, adjust the pH to 7.5-7.6.
To the ingredients as in nutrient broth, add 15 gm agar per litre. Dissolve the agar in nutrient broth and sterilize by autoclaving at 121°C for 15 minutes. Prepare plates and slopes as required.
Nutrientbroth 900ml Glucose (10% solution) 100 ml
Dissolve 9 gm glucose in distilled water and sterilize by tyndallisation.
Add l00 ml of the glucose solution to 900 ml of sterile nutrient broth.