Add disodium hydrogen phosphate to dissolve.
Add phenol red and adjust pH to 8.4.
Distribute 6 ml in universal containers (screw -capped
bottles of 30 ml capacity). Autoclave at 115°C for
Loeffler serum medium
Dissolve glucose in nutrient broth and sterilize at 121°C for 15 minutes.
Add serum aseptically.
Mix thoroughly but gently, avoiding froth formation.
Distribute in sterile test tubes or quarter ounce screw-cap bottles.
Inspissate the medium in a slanting position in a water inspissator at 82°C for two hours.
In the absence of an inspissator, the medium may be coagulated by standing over the top of a steam sterilizer for 6-7 minutes.
Blood tellurite agar