Dissolve the ingredients and adjust the pH to 7.6. Distribute in 100 ml quantities in a bottle and autoclave at 121°C for 15 minutes.
Glycerolated blood tellurite mixture
Sterile defibrinated sheep blood 14 ml Sterileglycerol 6 ml Sterile potassium tellurite solution (1% in water) 4 ml
Sterilize the glycerol in hot air oven at 160°C for 60 minutes and the tellurite solution by autoclaving at
115°C for 20 minutes. Mix the ingredients in a sterile flask, incubate for 1-2 hrs. at 37°C, then refrigerate.
Haemolysis is complete after 24 hrs. The mixture keeps well in a refrigerator. One per cent solution of
good quality tellurite is sufficient but 2% of some batches may be required.
Preparation of complete medium
Glycerolated blood tellurite mixture 24 ml Agar base 100 ml