Melt the agar, cool to 45°C, add blood and tellurite and pour in sterile petri dishes.
M1. A-1 Medium Tryptone 20 g Lactose 5 g NaCl 5 g *Triton X-100 (Rohm & Haas) 1 ml Salicin 0.5 g Distilled water 1 liter
Dissolve ingredients in 1 liter distilled water. Adjust pH to 6.9 ± 0.1. Dispense 10 ml portions of single strength broth into 18 x 150 mm tubes containing inverted fermentation vials. For double strength broth, use 22 x 175 mm tubes containing inverted fermentation vials. Medium may be cloudy before sterilization. Autoclave 10 min at 21°C. Store in dark up to 7 days. (Commercially available A-1 medium is unacceptable.)
M2. Acetate Agar Sodium acetate 2 g NaCl 5 g Mg SO4 (anhydrous) 0.2 g Ammonium phosphate1 g K2HPO4 1 g Bromthymol blue 0.08 g Agar 20 g Distilled water 1 liter