Beef extract 5 g Dextrose 5 g Na2HPO4 (anhydrous) 4 g FeSO4 (anhydrous) 0.3 g Bismuth sulfite (indicator) 8 g Brilliant green 0.025 g Agar 20 g Distilled water 1 liter
Mix thoroughly and heat with agitation. Boil about 1 min to obtain uniform suspension. (Precipitate will not dissolve.) Cool to 45-50°C. Suspend precipitate by gentle agitation, and pour 20 ml portions into sterile 15 x 100 mm petri dishes. Let plates dry about 2 h with lids partially removed; then close plates. Final pH, 7.7 ± 0.2 DO NOT AUTOCLAVE. Prepare plates on day before streaking and store in dark. Selectivity decreases in 48 h.
M14. Blood Agar Tryptone 15 g Phytone or soytone 5 g NaCl 5 g Agar 15 g Distilled water 1 liter
Heat with agitation to dissolve agar. Autoclave 15 min at 121°C. Cool to 50°C. Add 5 ml defibrinated sheep red blood cells to 100 ml melted agar. Mix and pour 20 ml portions into sterile 15 x 100 mm petri dishes. Final pH of base, 7.3 ± 0.2. Tryptic soy agar, tryptic soy