Na2HPO4 *2.5 g Distilled water 1 liter
Dissolve ingredients of Medium 1 in distilled water with gentle heat. Suspend ingredients of Medium 2 in distilled water and boil for 1 min to completely dissolve. For both Medium 1 and Medium 2, dispense broth into bottles or tubes for storage. Autoclave 15 min at 121°C. Final pH, 7.4 ± 0.2. Commercially available BHI is acceptable.
To prepare brain heart infusion agar, add 15 g agar to 1 liter BHI broth. Heat to dissolve agar before dispensing into bottles or flasks. Autoclave 15 min at 121°C.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
M19. Brilliant Green Lactose Bile Broth
Peptone 10 g Lactose 10 g Oxgall 20 g Brilliant green 0.0133 g Distilled water 1 liter
Dissolve peptone and lactose in 500 ml distilled water. Add 20 g dehydrated oxgall dissolved in 200 ml distilled water. The pH of this
solution Adjust distilled
should be 7.0-7.5. Mix and add water to make 975 ml. pH to 7.4. Add 13.3 ml 0.1% aqueous brilliant green in water. Add distilled water to make 1 liter. Dispense into