Dissolve ingredients in distilled water. Dispense 12-15 ml into tubes. Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2..
M22. Egg Yolk Emulsion, 50% Wash fresh eggs with a stiff brush and drain. Soak eggs 1 h in 70% ethanol. Drain ethanol. Crack eggs aseptically and discard whites. Remove egg yolks with sterile syringe or wide-mouth pipet. Place yolks in sterile container and mix aseptically with equal volume of sterile 0.85% saline. Store at 4°C until use. Egg yolk emulsion (50%) is available commercially.
M23. Heart Infusion (HI) Broth and Agar (HIA) (for Vibrio) Beef heart, infusion from 500 g 1 liter Tryptose 10 g NaCl 5 g
Dissolve ingredients and dispense into tubes. For halophilic Vibrio spp. add an extra 15 g NaCl/liter. Autoclave 15 min at 121°C. Final pH, 7.4 ± 0.2. For heart infusion agar, add 15 g agar/L and boil to dissolve before dispensing and sterilizing.Commercially available from Difco.
M24. Kligler Iron Agar Polypeptone peptone 20 g Lactose 20 g Dextrose 1 g NaCl 5 g Ferric ammonium citrate 0.5 g