Sodium thiosulfate 0.5 g Agar 15 g Phenol red 0.025 g Distilled water 1 liter
Heat with agitation to dissolve. Dispense into 13 x 100 mm screw- cap tubes and autoclave 15 min at 121°C. Cool and slant to form deep butts. Final pH, 7.4 ± 0.2.Commercially available from Difco, BBL, and Oxoid.For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%. M25. Lysine Decarboxylase Broth (Falkow) (for Salmonella) Gelysate or peptone 5 g Yeast extract 3 g Glucose 1 g L-Lysine 5 g Bromcresol purple 0.02 g Distilled water 1 liter
Heat until dissolved. Dispense 5 ml portions into 16 x 125 mm screw-cap tubes. Autoclave loosely capped tubes 15 min at 121°C. Screw the caps on tightly for storage and after inoculation. Final pH, 6.8 ± 0.2. For halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
M26. Lysine Decarboxylase (LDC) Medium (for Gram-negative nonfermentative bacteria) L-Lysine HCl 0.5 g