M32. Motility Test Medium (Semisolid) Beef extract 3 g Peptone or gelysate10 g NaCl 5 g Agar 4 g Distilled water 1 liter
Heat with agitation and boil 1-2 min to dissolve agar. Dispense 8 ml portions into 16 x 150 screw-cap tubes.Autoclave 15 min at 121°C. Final pH, 7.4 ± 0.2.
For Salmonella: Dispense 20 ml portions into 20 x 150 mm screw- cap tubes, replacing caps loosely. Autoclave 15min at 121°C. Cool to 45°C after autoclaving. Tighten caps, and refrigerate at 5-8°C. To use, remelt in boiling water or flowing steam, and cool to 45°C. Aseptically dispense 20 ml portions into sterile 15 x 100 mm petri plates. Cover plates and let solidify. Use same day as prepared. Final pH, 7.4 ± 0.2.For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
M33. Nitrate Broth Beef extract 3 g Peptone 5 g KNO3 (nitrite-free) 1 g Distilled water 1 liter
Dissolve ingredients. Dispense 5 ml portions into 16 x 125 mm tubes. Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.