Rehydrate and sterilize base as recommended by manufacturer. Agar and blood should both be at 45-46°C before blood is added and plates are poured. Commercial pre-poured sheep blood agar plates may be used.
M38. Shigella Broth Base Tryptone 20 g K2HPO4 2 g KH2PO4 2 g NaCl 5 g Glucose 1 g Tween 80 1.5 ml Distilled water 1 liter
Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.
Novobiocin solution. Weigh 50 mg novobiocin into 1 liter distilled water. Sterilize by filtration through 0.45 µm membrane. Add 2.5 ml concentrate to 225 ml base.
M39. Simmons Citrate Agar Sodium citrate*2 g NaCl 5 g K2HPO4 1 g NH4H2PO4 1 g MgSO4 0.2 g Bromthymol blue 0.08 g