Na2HPO4 11 g Distilled water 1 liter
Adjust pH to 7.8 ± 0.1. Dispense 15 ml portions into 20 x 150 mm screw-cap tubes. Autoclave 15 min at 121°C.
M42. Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar Yeast extract 5 g NaCl 10 g Peptone 10 g Ferric citrate 1 g Sucrose 20 g Bromthymol blue O.04 g Sodium thiosulfate·5H2O 10 g Thymol blue 0.04 g Sodium citrate·2H2O 10 g Agar 15 g Sodium cholate 3 g Distilled water 1 liter Oxgall 5 g
Prepare in flask at least 3 times larger than required volume of medium. Add ingredients to warm distilled water and heat to dissolve. Bring just to boil, and immediately remove from heat. DO NOT AUTOCLAVE. Cool to 50°C and pour into petri dishes. Dry the plates overnight or at 37-45°C before use.
M42. Triple Sugar Iron Agar (TSI)