M150. Trypticase Novobiocin (TN) Broth Trypticase soy broth 30 g Bile salts No. 3 1.5 g Dipotassium phosphate 1.5 g Novobiocin 20 mg Distilled water 1 liter
Dissolve all ingredients except novobiocin by heating and stirring; autoclave at 121°C for 15 min. Prepare stock solution of novobiocin by adding 20 mg monosodium novobiocin per ml of distilled water. Filter-sterilize. Make fresh stock each time of use, or store frozen at - 10°C in the dark (compound is light-sensitive) for not more than 1 month (half-life is several months at 4°C). Add 1 ml stock solution per liter of medium.
M43. Trypticase (Tryptic) Soy Agar Trypticase peptone 15 g Phytone peptone 5 g NaCl 5 g Agar 15 g Distilled water 1 liter
Heat with agitation to dissolve agar. Boil 1 min. Dispense into suitable tubes or flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2. For use with halophilic Vibrio