Dissolve BSA in PBS buffer. Aliquot and store at -20°C.
R8. Brilliant Green Dye Solution. 1% Brilliant green dye 1 g Distilled water (sterile) 10 ml
Dissolve 1 g dye in sterile water. Dilute to 100 ml. Before use, test all batches of dye for toxicity with known positive and negative test microorganisms.
R9. Bromcresol Purple Dye Solution. 0.2% Bromcresol purple dye 0.2 g Distilled water (sterile)100 ml
Dissolve 0.2 g dye in sterile water and dilute to 100 ml.
R10. Bromthymol Blue Indicator. 0.04% Bromthymol blue 0.2 g 0.01 N NaOH 32 ml
Dissolve bromthymol blue in NaOH. Dilute to 500 ml with distilled water.
R11. Butterfield's Phosphate-Buffered Dilution Water Stock solution KH2PO4 34 g Distilled water 500 ml